Current methods of euthanizing piglets are raising animal welfare concerns.Our experiment used a novel two-step euthanasia method, using nitrous oxide (N2O) for six minutes and then carbon dioxide (CO2) on compromised 0- to 7-day-old piglets.A commercial euthanasia chamber was modified to deliver two euthanasia treatments: the two-step method using
A nanomaterial life-cycle risk assessment (Nano LCRA) was conducted for second-generation functionalized cellulose nanomaterials (CNs) in five case studies, including applications in water filtration, food contact packaging (including as an additive and coating), Wade and food additives, to identify and prioritize potential occupational, health, co
The isolation and cloning of a random amplified polymorphic Wade DNA-polymerase chain reaction (RAPD-PCR) band specific for Pseudomonas syringae pv.phaseolicola race 1 allowed us to design a pair of primers that amplify 1.2-kb race 1-specific and 2.7-kb race 2-specific fragments, providing a rapid method for the identification of races by standard
BACKGROUND Knowledge on synanthropic phlebotomines and their natural infection by Leishmania is necessary for the identification of potential areas for leishmaniasis occurrence.OBJECTIVE To analyse the occurrence of Phlebotominae in gallery forests and household units (HUs) in the city of Palmas and to determine the rate of natural infection by try